منابع مشابه
Structural genomics plucks high-hanging membrane proteins.
Recent years have seen the establishment of structural genomics centers that explicitly target integral membrane proteins. Here, we review the advances in targeting these extremely high-hanging fruits of structural biology in high-throughput mode. We observe that the experimental determination of high-resolution structures of integral membrane proteins is increasingly successful both in terms o...
متن کاملStructural bioinformatics prediction of membrane-binding proteins.
Membrane-binding peripheral proteins play important roles in many biological processes, including cell signaling and membrane trafficking. Unlike integral membrane proteins, these proteins bind the membrane mostly in a reversible manner. Since peripheral proteins do not have canonical transmembrane segments, it is difficult to identify them from their amino acid sequences. As a first step towar...
متن کاملStructural prediction of membrane-bound proteins.
A prediction algorithm based on physical characteristics of the twenty amino acids and refined by comparison to the proposed bacteriorhodopsin structure was devised to delineate likely membrane-buried regions in the primary sequences of proteins known to interact with the lipid bilayer. Application of the method to the sequence of the carboxyl terminal one-third of bovine rhodopsin predicted a ...
متن کاملOverexpression of membrane proteins in mammalian cells for structural studies
The number of structures of integral membrane proteins from higher eukaryotes is steadily increasing due to a number of innovative protein engineering and crystallization strategies devised over the last few years. However, it is sobering to reflect that these structures represent only a tiny proportion of the total number of membrane proteins encoded by a mammalian genome. In addition, the str...
متن کاملA structural genomics approach to membrane transport proteins
The hydrophobic bilayer membrane that bounds cells is inherently impermeable to the great majority of hydrophilic solutes required for cell nutrition and to many of the waste products and/or toxins that must be excreted. Accordingly, the membrane contains proteins, the sole function of which is to catalyse the translocation of substrates through the membrane. In all types of cells, from microbe...
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ژورنال
عنوان ژورنال: Annual Review of Biophysics
سال: 2015
ISSN: 1936-122X,1936-1238
DOI: 10.1146/annurev-biophys-051013-023008